LAST PROJECTS
|
NN
|
Title of project
|
Requester
|
Data of execution
|
Role in the project
|
|
Research Centre of Molecular Diagnostics and Theraphy
|
|
1
|
Investigation of possibilities of biosensor creation of Hepatitus B (HBs) viruses detection and cells with sub-cells structures having receptors of biogenic amines and opioic substances.
Number "Ligand"
|
Ministry of USSR Health
|
1990-1992
|
Fellow
|
|
2
|
Creation of micro-gravimetric immunosensors based on monomolecular films of antigens
|
International Research and Development Project "Biosensors",
Institute of Chemo- and Biosensors, Munster, Germany
|
1992-1995
|
Fellow
|
|
3
|
Creation of gravimetric biosensors based on monomolecular films of antigenes.
Number 7-478
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1993-1995
|
Fellow
|
|
4
|
Development of detection system of stimulators of super dangerous diceases and toxines based on sensitive element's surface analysis by the scanning tunnelling and atomic-force microscopical methods for realization of the national and international control of biological objects activity.
Number "Otrazhenie-K"
|
Geneva Convention Committee at the President of Russian Federation
|
1994-1995
|
Fellow
|
|
5
|
2D crystallisation of soluble protein molecules using amphyphilic polyelectrolytes and LB technology
|
AKZO Nobel
Central Research, Belgium,
Dr. M.S.Leloux
|
1994
|
Fellow
|
|
6
|
Development of biosensor systems for pesticides analysis based on enzymes and physical amplification systems
|
International Research and Development Project "Biosensors",
Institute of Chemo- and Biosensors, Munster, Germany
|
1995
|
Fellow
|
|
7
|
Family of fiber-optics sensors for ecological and bio-technological monitoring.
Number "Optics"
|
Ministry of Sciences and Technologies, Russian Foundation of Technological Development
|
1997-2000
|
Fellow
|
|
8
|
High-sensitive bio-electro-catalytic sensors based on conjugated poly-enzymes systems.
Number "Electrode"
|
Ministry of Sciences and Technologies, Russian Foundation of Technological Development
|
1997-2000
|
Fellow
|
|
9
|
Development of biosensor methods for the analysis of organo-phosphorus compounds induced acute and delayed neuro-toxicity.
Number 1055.2
|
International Science and Technology Centre
|
2000-2002
|
Project Manager
|
|
10
|
Immuno-sensors based on magneto-resistance technology
Number 1548
|
International Science and Technology Centre
|
2000-2002
|
Fellow
|
|
Bio-reactor, Ltd
|
|
1
|
Development of micro-gravimetric methods of low-molecular-weight antigenes analysis
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1992
|
Fellow
|
|
2
|
Investigation of physico-chemical properties of antigenes monomolecular films by the methods of "photobleaching" and tunneling microscopy. Development of model sample of device.
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1992
|
Fellow
|
|
3
|
Investigation of behaviour of piezzo-electric resonators in liquid
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1994
|
Fellow
|
|
4
|
Investigation of possibilities of creation of flow-injection systems based on piezzo-electric resonators
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1994
|
Fellow
|
|
5
|
Optimisation of physical enhancement system for purposes of paper immuno-analysis
|
Research Counsel on the Problem "Engineering enzymology", Russian State Research and Development Program "New methods of bioengineering"
|
1995
|
Fellow
|
|
6
|
Development of sensor electrodes contained conjugated enzymatic system for high-sensitive measurement device of inspector for determination of phosphorous-organic substances.
Number "Introscope B"
|
"Laboratory of gas-analytical developments", Ltd. of State Research Institute "Chimanalyt", St.Peterburg
|
1995
|
Fellow
|
|
Molecular Technology and Materials, Ltd.
|
|
1
|
Development of industrial technology and optimisation of operating parameters of biological analysis of Hepatitus B (HBs) viruses
|
Association of economical activity "Iskra", Lugansk, Unraine
|
1991-1992
|
Project manager
|
|
2
|
Development of thin films creation technology for immune and receptors bio-sensors on morphine
|
17th Drug Moscow hospital
|
1991-1992
|
Project manager
|
|
3
|
Development of synthesis methods of special polymers and investigation physical characteristics of protein-polymer complexes
|
Research Centre of Molecular Diagnostics and Theraphy
|
1992
|
Project manager
|
|
4
|
Amperometric detection of morphine in biological liquids
|
17th Drug Moscow hospital
|
1993
|
Project manager
|
|
5
|
Investigation of producing methods and structure of films from cells and viruses antigens and possibility of creation various types of detecting elements
|
Association of economical activity "Iskra"
|
1992
|
Project manager
|
|
6
|
Development of immobilisation methods of enzymes on conductive surfaces
|
Research Centre of Molecular Diagnostics and Theraphy
|
1993
|
Project manager
|
|
7
|
Investigation of biosensor analysis detection of morphine in biological liquids
|
17th Drug Moscow hospital
|
1993
|
Project manager
|
|
8
|
Finalisation of biosensor detection process of morphine in biological liquids
|
17th Drug Moscow hospital
|
1994
|
Project manager
|
|
9
|
Development of production technology of sensitive elements for STM/AFM based on molecular films of antibodies
|
Research Centre of Molecular Diagnostics and Theraphy
|
1994-1995
|
Project manager
|
|
Moscow State M.V.Lomonosov University
|
|
1
|
Kinetic aspects of metal phase forming on the crystal surface of silver halides
Number 96-03-32263a
|
Russian Foundation of Basic Research
|
1996-1998
|
Fellow
|
|
2
|
Theoretical and experimental investigations of nano-structures on supports.
Number 99-03-32-373a
|
Russian Foundation of Basic Research
|
1999-2000
|
Fellow
|
|
3
|
Biosensors for express detection and discrimination of ultra low concentrations of organophosphorous neurotoxins
Number 11003
|
IPP/CRDF
|
2000-2001
|
Fellow
|
|
|
|
|
|
|
|
FUTURE PROJECTS
Project 1 “Preparing of small colloidal particles of noble metal with extremely small diameter”
Development of preparation technique of Au, Ag, Pt, Pd (maybe Cu, Ni) colloidal particles with the size less 10 nm using wet chemical synthesis with following reductors: N2H4.H2O, N2H4.BH3, NaBH4, HCHO, NaH2PO2, (CH3)2NH.BH3, (C2H5)2NH.BH3, methol, pyrogallole a.a. (It is very important to protect from producing of boron compounds in the metals).
Media : water, alcohol.
Protective polymers: Polyvinyl alcohol, gelatin, polyethyleneglycole, polyvinylpyrollydone, a.a.
Stabilizations: surfactants, pyridine, citrate ions, a. a.
Investigation: type reductor, pH of solution, potential of solutions and particles, size of colloidal particles, spectrum of absorption, optimization of preparation for creation of minimal size (temperature, solvent, polymer or protein protective media) stability of colloidal systems during a rather long time. Creation of hybrid particles like Au/Ag, Pd/Ni with the help of physical development.
Project 2 “Sonochemical preparing of small colloidal particles of metals and oxides”
Development of preparation technique of colloidal particles with the size less 300 nm using sonochemical synthesis for metals and their oxides.
Types of metals: Pt, Ni, Fe, Co, Cu, W, a. a.
Media: hydrocarbons, alcohols, water.
Atmosphere: inert gases (Ar, N2), oxygen O2. In the inert atmosphere we can create metal particles, in oxygenic one the particles of their oxides.
Substances for sonochemical synthesis: carbonyls, diketonates and other easily evaporable compounds of metals.
Stabilizations: polyethyleneimine, surfactants, pyridine, citrate ions, a. a.
Investigations: influence on the particles’ dispersity of type of metals, media, stabilizations, power and frequency of ultrasonic oscillator, temperature and duration of process. Investigation of stability in organic (oxygen-less) solvents, water, on air. Creation hybrid particles by consecutive synthesis.
Project 3 “Using of small colloidal particles as markers of biologically active places onto the cells’ surface”
Producing of colloidal particles of noble metals of different sizes, labeled their with the biologically active substances, investigation of topography of active places onto the cells’ surface with the help of such colloidal particles with high electron density as markers and by using of unique carbon replica technique. Simultaneously investigation of several types of biological active places with the help of several sizes or several types of colloidal particles.
Types of metals: Au, Pt, Ag.
Size of particles: 10 nm, 5 nm and less.
Biological substances: antibodies, receptors, different types of cells’ proteins, a. a.
Investigation of processes: phagocytosis, erythrocytes-macrophages or lymphocytes-macrophages rosettes, antigen-antibody interactions, detection of pathogenic bacterium a. a. Investigation of 3D surfaces of cells.
Additional task: Creation of conductive nano-fibers or nano-tubes from self-assembled protein labeled gold colloidal particles.
Project 4 “Langmuire films of amphiphilic polymers as protective layers by forming and transferring of active films of biological substances”
Development technique preserving of biological molecules against the destruction effect by transforming through the water surface by immobilization onto the substrate. We propose the Langmuire technique and protective layers of amphiphilic polymers.
Types of polymers: polyethyleneimine, polyvinyl-pyrrolidone, polyacids (like polyacrilic acid) modified with hydrocarbons chains.
Types of biological substances: enzymes, antibodies, receptors a. a.
Investigation of processes: forming of Langmuire films, isotherms of adsorption, temperature and duration of process, process of interaction between monomolecular film and proteins molecules. Investigation of protein-polymer interaction process in volume and onto the water sub-phase surface.
Project 5 “Creation of small colloidal particles for new generation of biosensors based on BARC format”
Synthesis colloidal particles covered by polymeric layers for protect from media, for easy labeled by proteins, for creation of stabile suspension in the physiology liquids for Beads ARray Counter format.
Particles from: magnetite, gold or silver.
Detection by: gigant magnetic resistance effect sensors, transmitted electron microscope.
Investigations: process of polymerization onto the magnetic particles surface and different types of inclusion of particles into the polymer core, stability of suspension, charge of particles, adsorption of proteins onto the particles’ surface, stability of biological activity. Increasing of stability with the help of different types of polymers like branched and charged polycations or polyanions.
Project 6 “Langmuire 2D crystal films of biological substances”
Creation of 2D films from proteins for electron microscopy investigation of structure and chains’ conformation.
Investigation: influence the various types of drug or perfume substances onto the protein 2D structure.
Using technology: Langmuire films creation, adhesion to lipid film, creation of amphiphilized proteins.
We have not enough of experience in this area –the creation only 2D films of ferritine.
Project 7 “Investigation of new phase generation and real structure of point-defects in pure crystals or in the crystals with special entered impurities”
Purpose of project: investigation of new phase (natural and specially entered) generation process in the crystals using of vacuum decoration methods of point defects with colloidal particles on electron microscope level.
Crystals: ultra-fine-pure KCl, NaCl, ZnO, LiF, SiO2 or crystals with special entered components.Impurities: natural OH-; special entered: Cation2+, Anion2-; F-centers by γ-irradiation and addition coloring in the vapors of metals.
Types of colloidal particles: Au, Ag, AgCl, AgBr, PbS, Ga.
Project 8 “Investigation of formation of new silver phase during photolysis and physical and chemical developments in ultra-small-grained silver halide micro crystals and during additional treatment”
Purpose of project: investigation of process of formation of new colloidal silver phase and oligoatomic silver clusters in ultra-fine-grained AgBr crystals of transparent Lippmann photoemulsions by photolysis, chemical and physical development.
Crystals: AgBr with size of 0 to 200 nm.
Colloidal particles of Ag with size of 3 to 30 nm.
Clusters of Ag of 1 to 10 atoms.