Example evolution of cow:
The idea is to have real models of the evolution of the creature that
people can touch, sit on etc. They may have little signs next to them like
they have in botanic gardens.
EXAMPLE: The evolution of corn
Approximately five major regions of the genome are responsible for the
transition from a teosinte-like ancestor to modern corn.
Two of these regions have been identified recently by John Doebley et
al.
(1995).
One maps to a gene (teosinte branched 1) that affects whether a flower
becomes male (tassel) or female (corn cob) and affects the length of
side
branches.
A second region also affects length of side branches.
By making appropriate crosses between maize and teosinte, the authors
were able to place alleles within these regions from maize into a teosinte
genetic background.
For every trait that the regions affected, the alleles were less dominant
in
teosinte than they were in maize.
"This change in gene action could have resulted from selection during
the
domestication process for modifier loci that enhanced the expression
of the
trait in the heterozygote."
- Doebley et al. (1995)
NEW EVIDENCE FOR CORN'S ANCESTRY COULD LEAD TO INSECT AND DROUGHT RESISTANT
CROPS
DURHAM, N.C. -- Scientists continue to debate the
ancestry of domesticated
corn. But Duke University researcher Mary Eubanks
said she has mounting
evidence that corn emerged from the interbreeding
of two different wild
American grasses. Those grasses are Tripsacum dactyloides,
also known as
Eastern gamagrass, and Zea diploperennis, a perennial
variety of teosinte.
By successfully crossing them for the first time,
Eubanks has produced
fertile offspring that closely resemble the earliest
known samples of
primitive domesticated corn, she said in a recent
interview. Patented lines
of her crosses are fertile over several generations
and carry genetic
traits that are "missing links" in corn's evolution,
Eubanks adds in the
latest issue of the journal Theoretical and Applied
Genetics. That article
updates experiments originally described in the
June, 1995 issue of
Economic Botany. She will report on her work again
at an August meeting of
the American Institute of Biological Sciences in
Montreal. If Tripsacum is
indeed a corn ancestor, then breeders could take
advantage of some of the
special traits of that hardy ubiquitous plant, the
Duke botany research
scientist said. Indeed, Eubanks has crossed her
lines with modern corn to
produce hybrids that share Tripsacum's resistance
to the corn rootworm.
That's the root-eating juvenile -- or larval --
stage of an insect that
costs corn farmers $1 billion a year in crop losses
and pesticide
expenses. In preliminary experiments at Duke's Phytotron,
a high-tech
greenhouse where she rents space, Eubanks found
far less root damage and
far fewer larvae in her experimental hybrids than
occurs in normal
commercial corn. "We're using the western corn rootworm,
which is the most
serious pest in the Midwest," she said. "It's an
incredible problem and a
big expense for corn growers. Because they don't
know where they are going
to have an outbreak, they now have to treat all
their fields with extremely
toxic chemicals." Tripsacum is drought resistant
too, and Eubanks plans to
test her experimental hybrid for that trait as well.
Moreover, her
Tripsacum-teosinte hybrids are perennials, and could
conceivably be
exploited to produce a perennial variety of corn,
she added. All of today's
commercial corn lines are annuals, meaning that
their seeds must be planted
anew each year. Eubanks holds four patents on various
Tripsacum-teosinte
crosses developed during the past decade. She is
also president of Sun
Dance Genetics, a Durham, N.C. research company.
Corn is "an anomaly in the
botanical kingdom," Eubanks said. "You have this
tremendous ear. The ear
has hundreds of kernels, held together on a rigid
stalk and enclosed by a
husk so they're easy to harvest and dry and store.
It provided a wonderful
food for the original inhabitants of the Americas
once they began
cultivating it." But the earliest known versions
of corn have far smaller
ears that bear fewer and tinier kernels, providing
silent testimony to the
corn genome's great adaptability. Scientists are
in agreement on the
ancestry of the Old World's simpler staple grains
-- oats, rye and wheat.
But the origins of the New World's major grain remain
cloaked with mystery.
That's true even though "this is one of the most
well studied organisms."
she said. With a Ph.D. in anthropology, Eubanks
would seem an unlikely
person to pursue research into the molecular biology
of corn genes. But,
while still in graduate school at the University
of North Carolina, she met
Paul Mangelsdorf, a premier corn researcher who
had retired from Harvard to
surrounding Chapel Hill. As an anthropologist, Eubanks
was already
investigating the corn images that pre-Columbian
Central Americans
frequently incorporated in their pottery. And she
said that interested
Mangelsdorf because native American pottery makers
pressed real ears of
corn into the wet clay to form botanically accurate
molds. That meant her
pottery studies could give him vital clues about
how domesticated corn
looked many centuries before the Spanish conquest.
So her research trips to
Mexico and Peru aided Mangelsdorf's continuing work.
In turn, Mangelsdorf's
passion quickly rubbed off on her. Eubanks began
studying biology and
performing botanical research at Duke, Indiana,
North Carolina State and
Vanderbilt universities. Scientists, including herself,
now agree that
teosinte is an ancestor of corn, Eubanks said. Native
to Mexico and
Guatemala, it features corn-like leaves and a tall
stalk crowned by
corn-like tassels. It also has hard seeds that line
up
in a single row -- called a "spike." However, Mangelsdorf
once believed one
of corn's true ancestors was Tripsacum, a grass
that ranges throughout
North and South America. "It's a pervasive weed
around here," Eubanks said.
"It's on roadsides, railroad tracks and
bridges." The Tripsacum plant resembles corn less
than teosinte does. But
both grasses grow single rows of grain on spikes.
And both feature separate
male and female flowers on the same plant -- just
like corn. Moreover,
Tripsacum occasionally produces paired kernels,
a distinctive feature of
corn linked to the evolution of corn's multiple
rows. In contrast to
teosinte, Tripsacum's kernels are also easy to remove
from their hard
fruitcases, making them accessible as a wild food.
And "they are highly
nutritious and delicious," Eubanks said. Based on
breeding experiments in
the 1930s, Mangelsdorf and other researchers postulated
that the earliest
true corn was derived from a cross between Tripsacum
and a now-extinct wild
corn. Under that view, annually growing teosinte
-- the only kind then
known -- was a later offspring rather than an ancestor.
Archeological
evidence in Central America backed up Tripsacum's
role, Eubanks said. In
the 1960s, Mangelsdorf and colleagues found the
most ancient known
preserved corn samples, believed to be more than
5,000 years old, within
dry caves in Mexico's Valley of Tehuac‡n. The earliest
samples of teosinte
have been dated to 1800 B.C. And the
earliest evidence for Tripsacum, found at Tamaulipas
Mexico, predates that
teosinte by about 500 years. Nevertheless, Mangelsdorf
joined the teosinte
bandwagon in the 1980s after other researchers discovered
Zea diploperennis
growing on the threshold of extinction in the mountains
of Jalisco, Mexico.
Rescued from the wilds, lines of that perennial
teosinte are now being
maintained for research purposes. Mangelsdorf's
new hypothesis, which he
held until his death, said annual teosinte resulted
from a cross between
perennial Zea diploperennis and an extinct line
of early corn. Domesticated
corn then arose from crosses between that corn and
the new annual
teosintes. Most other experts believe natural genetic
mutations actually
caused the spikes of some wild teosinte plants to
directly evolve into the
first primitive ears of corn, Eubanks said. Under
that hypothesis, Native
Americans then improved the first corns through
accidental or intentional
breeding efforts. But Eubanks maintains that Tripsacum
is corn's other
ancestor plant. And the discovery of perennial teosinte
is helping her
prove that hypothesis, she said. Her initial breeding
experiments showed
that -- unlike any other teosinte -- Zea diploperennis
can be successfully
crossed with Tripsacum to produce a fertile hybrid.
Previous attempts to
breed Tripsacum with any other teosinte varieties
had failed. Similarly,
previous attempts by breeders to cross Tripsacum
with corn always resulted
in sterile plants. But Eubanks found she could also
successfully breed corn
with some of her Tripsacum and Zea diploperennis
crosses. Since fertile
offspring implies genetic similarities among plants,
Eubanks believed her
experiments rekindled the notion that Tripsacum
was a forebear of corn.
Other scientists initially didn't accept her hypothesis.
"It questioned
accepted dogma," she said. "I had difficulty convincing
the scientific
community that I even had a hybrid between Tripsacum
and the perennial
teosinte. They said I just had teosinte that was
contaminated with corn,
and that it was impossible to cross teosinte with
Tripsacum." Eubanks said
she continued in the face of criticism because she
knew her work had
already been validated by the U.S.
Patent Office's rigorous documentation process.
She also persisted because
of her plants' potential contributions to agriculture
and human welfare,
she added. Seeking further proof, Eubanks used molecular
genetic analysis
to show that traits from both plant types were present
in the offspring.
She worked with key heritable DNA markers that other
scientists --
including her principal detractor -- had previously
identified as being
crucial in the evolution of domestic corn. Her new
report in Theoretical
and Applied Genetics shows some of those markers
have now been "stably
inherited" for three generations, she said. Eubanks
is now embarked in an
expanded study of the complex genetic relationships
between teosinte,
Tripsacum, primitive corns and other related New
World grasses. The
National Science Foundation's Small Business Innovation
in Research program
is funding Eubanks' research on corn rootworm resistance.
She is conducting
her evolutionary studies of corn and related grasses
under an Andrew P.
Mellon fellowship in plant systemics at Duke. She
has also supported her
work from earnings as a self-employed consultant,
and by teaching advanced
high school biology at the North Carolina School
of Science and Mathematics
and Durham Academy. With funding from the National
Geographic Society, she
is now completing a book about the interdisciplinary
archaeobotanical
studies she did in the 1970s of corn impressions
on Mexican and Peruvian
pottery. The book, called Corn in Clay, will be
published by the University
of Florida Press. "There are two Peruvian pieces
in the collection here at
the Duke Art Museum," she noted. "There is one that
depicts a rat eating an
ear of corn. Then there is one that represents a
basket of corn." The first
piece is "somewhat problematic," Eubanks said. The
corn casts it carries
have more rows and larger kernels than any Latin
American race of corn that
exists today. "We think it may represent an extinct
race," she added.
http://www.dukenews.duke.edu/research/corn.html
June 4, 1997 Contact: Monte Basgall (919) 681-8057
[email protected]